Mature to sh (m2sh)
1. Sequence
Sequence of mature microRNA:
0
nucleotides typed in yet.
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2. Optional parameters
Introduce missmatch for Drosha.
Position of mature
1 (5' arm)
2 (3' arm)
Print pcr oligos.
Change the head sequence to
Change the tail sequence to
Change the loop sequence to
Change PCR5' Enzyme seq
user specified 5' end of 5' primer for PCR. Default is CAGAAGGCTCGAGAAGGTATAT (XHO1)
Change PCR3' Enzyme seq
user specified 5' end of 3' primer for PCR. Default is CTAAAGTAGCCCCTTGAAT (ECOR1)